Bio-Rad iMark Microplate Absorbance Reader - Manual (Page 28)

iMark Microplate Absorbance Reader Instruction Manual
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Editing the Kinetic Protocol
Select “Protocol” in the Edit Menu and press Enter. The Kinetic Protocol menu will appear on
the screen if the current active protocol type is Kinetic.
Reading speed
[Fast mode]
Step mode
Speed
» ■Reading ·Speed
·Inter.
·Shaking
·Ph.mode
Kinetic Mode
Kinetic Protocol Menu
» ■Mode ·Report
·Calc. ·Kit name
·Mapping
·Assay
This menu screen can be accessed by selecting “Mode” in the Kinetic Protocol menu
above.
Number of Reading
=
20 (2-30)
Start Delay time
=
25 sec(0-999)
Reading
Number of Reading
= 2
Start Delay time
=
25 sec(0-999)
Number of Readings and Start Delay Time Setting
Press the Up or Down arrow key to change the number.
Enter the appropriate numbers with the numeral keys, and press Enter to complete
the field entry. The system will return to the Kinetic Edit Menu.
If GALT is the current assay type, the number of reading fields is fixed at 2.
Press the Start/Stop key to set the Start Delay time, enter a value and press Enter,
until the reading starts.
1->2:» ■ 100 sec
2->3: 100 sec
3->4: 100 sec
↓4->5: 100 sec
Inter.
Use same interval
between all reads?
» ■Yes No
Setting Reading Intervals
The system prompts the operator to set a reading interval before each plate run.
Move the cursor to the desired selection “Yes” or “No” and press Enter. The
system generates the interval entry screen.
Move to the active entry field by pressing the Up and Down arrow keys, and enter
appropriate numbers using numeral keys. Press Enter to complete all field entries.
The system will return to the Kinetic Mode menu.
Photometric: Dual
Measurement: 405 nm
Reference: 655 nm
Ph. mode
Photometric Mode Setting
When the “Photometric” field is activated, pressing the Right arrow key will
switch the field entry: “Single” <->”Dual”.
Pressing the Up or Down arrow keys will move the cursor to the next entry field.
Pressing the Right arrow key when the wavelength field is activated will change
the wavelength to the next available selection installed in the reader.
Press Enter to complete all field entries.
Shaking parameters
Before:
Every read
Speed: Mid
Time: 999 sec
Shaking Plate Shake Parameters
When the “Before” field is activated, pressing the Right arrow key will change
the field entry: “Every”->”--”->”First”->. When the “Speed” field is activated,
pressing the Right arrow key will change the field entry: “Mid”
->”Hi”->”Low”->.
When the “Time” field is activated, enter the time value for plate shaking period
using the numeral keys.
Press Enter to complete all field entries.
Selecting Reading Speed
Use the Up or Down arrow keys to switch the selection. Press Enter to complete
the selection.
Edit Menu
» ■Protocol ·Lab.name
·Security •SaveSTDC
·Filters •Printer
·Date set
This menu screen can be accessed by pressing the Edit key in the MAIN screen.