Bio-Rad iMark Microplate Absorbance Reader - Manual (Page 34)

iMark Microplate Absorbance Reader Instruction Manual
28
Coefficient for Cutoff Formula 12
The Numerical keys and the Decimal point key are used to enter
the coefficient "k1" and "k2" and gray zone values. The Up and
Down arrow key moves the cursor to the desired cutoff formula.
The Enter key sets the all values of the cutoff formula and returns
to the previous screen.
NOTE:
"CNx" is the mean absorbance value of Negative control
wells, "CPx" is the mean absorbance value of Positive control wells,
and "COx" is the mean absorbance value of Cutoff control wells.
These wells are defined on the plate map setting. The cutoff value
is calculated using the defined formula with the defined values and
coefficients.
d. Cutoff Ratio
Ratio to Calibrator
In this screen, there are three items for setting the ratio to calibrator. The Up and Down
arrow key moves the cursor to the desired item in the menu list. The Enter key seals the
selection and resumes to the next setting screen.
Concentration of Calibrator
The Numerical keys and the Decimal point key are used to enter
the calibrator concentration value. The Enter key sets the value and
returns to the previous screen.
Unit of Calibrator
Seventeen standard units are supported. The unit marked with
“[ ]” is the current selection.
The setting procedure is the same as the unit selection of Standards.
Cutoff Value Setting
This menu screen selects Single or Ranged cutoff type. The cutoff
type marked with “[ ]” is the current selection. The Enter key
marks the selection and continues to the next setting screen
Single Cutoff Control
The Numerical keys are used to enter the Positive cutoff
concentration value and gray zone value. The Enter key sets these
cutoff ratio values and returns to the previous screen.
Ranged Cutoff Control
The Numerical keys are used to input the Positive and the Negative
cutoff concentration values. The Enter key sets these cutoff ratios
and returns to the previous screen.
NOTE: The calibrator concentration value is used to convert the
absorbance value of each well into a concentration value. The ratio
of Concentration/Absorbance of Calibrator well is applied to the
conversion. The cutoff uses defined Positive and Negative values to
the converted concentration value of each well.
Input coefficient
k1 =
0.01 (0.01-10)
k2 = 10.0 (0.01-10)
Gray zone: 50 %(0-99)
k1 * CNx + k2 * CPx
Ratio to Calibrator
»
■Concentration
•Unit
•Cutoff value
Concentration: 9999
unit: mol/l
↑»■ 01: ug/dl
02: ng/ml
03:[ pg/ml]
↓ 04: pm/ml
» ■Positive >= 9999
Gray zone: 50 %
Unit: mol/l
» ■Positive >= 9999
Negative < 9999
Negative
Unit: mol/l
» ■Ranged cutoff
[Single cutoff]
Concentration
Unit
Cutoff value
Single cutoff
Ranged cutoff