Bio-Rad iMark Microplate Absorbance Reader - Manual (Page 33)

iMark Microplate Absorbance Reader Instruction Manual
27
b. Cutoff Control
Selection of Single/Ranged Cutoff Control
This menu screen selects Single or Ranged cutoff type. The cutoff type marked with
“[ ]” is the current selection. The Enter key seals the selection and resumes to the next
setting screen.
Single Cutoff Control
The Numerical keys are used to enter the gray zone value. The unit
is fixed to "Abs". The Enter key sets the value of the cutoff control
and returns to the previous screen.
Ranged Cutoff Control
There is no parameter to set for the ranged cutoff control.
The Enter key returns to the previous screen.
NOTE: "CNx" is the mean absorbance value of Negative control
wells and "CPx" is the mean absorbance value of Positive control
wells. These wells are defined in the plate map setting. The cutoff is
done with the Positive and Negative value to the absorbance value
of each well.
c. Cutoff Formula
Selection of Formula
This menu screen selects a formula for cutoff calculation. The formula marked with
“[ ]” is the current selection. The Up and Down arrow keys move the cursor to the
desired formula type. The movement of the cursor follows the scroll of this screen. The
Enter key marks the selection and advances the user to the next setting screen.
The supported formula types are:
1. k * CNx
2. k * CPx
3. k * COx
4. CNx / k
5. CPx / k
6. COx / k
7. k + CNx
8. k + CPx
9. k + COx
10. k*CNx + CPx
11. ( CNx + CPx ) / k
12. k1*CNx + k2*CPx
Coefficient for Cutoff Formula 1 to 11
The Numerical keys and the Decimal point key are used to enter
the coefficient "k" and gray zone values. The Up and Down arrow
key moves the cursor to the desired parameter of the cutoff formula.
The Enter key sets all values of the cutoff formula and returns to
the previous screen.
Positive >= CNx
Gray zone:
50 %
Unit: Abs
Positive >= CPx
Negative < CNx
Unit: Abs
Enter key to accept
» ■Ranged cutoff
[Single cutoff]
Single cutoff
Ranged
Input coefficient
k =
0.01 (0.01-10)
Gray zone:
50 %(0-99)
k * CNx
k * CPx
k * COx
CNx / k
CPx / k
COx / k
k + CNx
k + CPx
k + COx
k * CNx + CPx
(CNx + CPx) / k
↑ »■CNx / k
•CPx / k
•COx / k
↓ •k + CNx
Select formula
»
■[k*CNx ]
•k*CPx
↓ •k*COx
↑ »■k + CPx
•k + COx
•k*CNx + CPx
↓ •(CNx + CPx)/ k
↑ »■k1*CNx + k2*CPx
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