Cutoff setting procedure, Cutoff setting menu, A. cutoff constant – Bio-Rad iMark Microplate Absorbance Reader User Manual

Page 32: Selection of single/ranged cutoff constant, Single cutoff constant, Ranged cutoff constant

iMark Microplate Absorbance Reader Instruction Manual

Cutoff Setting Procedure

Cutoff Setting Menu

This menu is entered by choosing "Cutoff" in the End Point Protocol Menu. It is used to set the
cutoff type. The cutoff type marked with “[ ]” is the current selection. The Up and Down
arrow keys move the cursor to the desired item in the menu list. When the desired parameters
have been selected, press Enter twice to accept the selection and to continue to the next screen.
"Not use" means that the reader does not generate the cutoff report.

Four types of cutoff are supported:

1. Cutoff Constant
2. Cutoff Control
3. Cutoff Formula
4. Cutoff Ratio

a. Cutoff Constant

Selection of Single/Ranged Cutoff Constant

This menu selects Single or Ranged cutoff type. The cutoff type marked with “[ ]” is
the current selection. The Enter key seals the selection and resumes to the next setting
screen.

Single Cutoff Constant

The Numerical keys and the Decimal point key are used to enter
the positive and gray zone values. The Right arrow key is used to
select the unit. The Up and Down arrow keys move the cursor to
the desired parameter of the single cutoff. The Enter key sets all
values and selection of the cutoff constant and returns the user to
the previous screen.

Ranged Cutoff Constant

The Numerical keys and the Decimal point key are used to enter
the positive and negative values. The Right arrow key is used to
select the unit. The Up and Down arrow key moves the cursor to
the desired ranged cutoff parameters. The Enter key sets all values
and selection of the cutoff constant and returns to the previous
screen.

NOTE:

Two available Cutoff constants are “Absorbance” and

“Concentration”. If "Abs" is chosen, the Absorbance cutoff will be
done with the cutoff constant parameters defined and the
absorbance report is generated. For other units defined in the curve
fit setting, the Concentration value using the curve fit and then
cutoff is done with the cutoff constant parameters defined and the
concentration value of each well. It is necessary to set the curve fit
parameters.

■Not usex •Ratio

[Const]
•Contrl
•Formula

■Positive >=

9999

Gray zone:

Unit:

mol/l

■Positive >=

9999

Negative <

1999

NegativeUnit:

mol/l

■Ranged cutoff

[Single cutoff]

Single cutoff

Ranged