Bio-Rad iMark Microplate Absorbance Reader - Manual (Page 16)

iMark Microplate Absorbance Reader Instruction Manual
10
Report Types
Two types of reports can be generated; End-point protocol, and Kinetic protocol. Each of these and their subtypes
are described below. After these descriptions, there are flow diagrams that show how to access specific choices.
End-point Protocol Reports
Nine types of End-Point Protocol reports can be generated: raw data, absorbance, limit, matrix, cutoff, curve fit,
concentration, difference, and TeSeE screening reports.
1. Raw data report
The Raw data report is the uncorrected absorbance values (without blank subtraction). In single-wavelength
mode, the reported value is the measured absorbance. In dual-wavelength mode, the reported value is the
difference between the uncorrected readings taken with the measurement filter and with the reference filter.
2. Absorbance report
The Absorbance report is the blank-corrected absorbance values. The mean absorbance value of all of the wells
designated as assay blanks is calculated and then subtracted from all 96 values of the raw data set to produce the
Absorbance report.
Abs = Raw – Blank mean
Blank mean = X/n
S.D. = [{X^2 – n*(Blank mean)^2}/{n-1}]^1/2
Where:
S.D. = Standard deviation
X = Sum total of the raw absorbance for each blank
X^2 = Sum total of the squared raw absorbance for each blank
n = Number of blanks
3. Limit report
The Limit report provides a qualitative YES/NO report. Wells with blank-subtracted absorbance values between
the upper and lower limits are represented with an asterisk (*), wells with absorbance values below the lower
limit by minus signs (-), and wells with absorbance values greater than the upper limit by positive signs (+).
4. Matrix report
The Matrix report provides a qualitative report of the relative magnitude of the absorbance values on the plate.
The absorbance range defined by the upper and lower limits is divided into 10 equal partitions, numbered 0
through 9. The blank-subtracted absorbance value of each well is classified according to the partition of the
matrix to which it corresponds, and is reported as a signal digit. Wells with absorbance values greater than the
upper limit are reported by plus signs (+), and wells with absorbance values less than the lower limit by minus
signs (-).