Bio-Rad iMark Microplate Absorbance Reader - Manual (Page 38)

iMark Microplate Absorbance Reader Instruction Manual
32
Mode Setting Procedure
Mode Setting Menu
This menu screen is entered by choosing the "Mode" in the End Point Protocol Menu screen or
the Kinetic Protocol Menu screen. It is used to set the plate reading conditions. The Up and
Down arrow keys move the cursor to the desired item in the menu list. The Enter key seals the
selection and resumes to the next setting screen.
a. Set Photo Mode
Photo Mode
In this screen, the user chooses Single or Dual wavelength measurement and assigns the
measurement and reference (for dual wavelength measurement) filters. The assignment
of the reference filter appears for dual wavelength measurement.
The Up and Down arrow keys move the cursor to the desired parameter. The Right
arrow key chooses Single/Dual wavelength measurement and selects the installed filter
on the "Filters" of Edit Menu screen. The Enter key seals the selection and returns to the
previous screen.
b. Set Shaking
Shaking
In this screen, the user selects/deselects shaking function, sets the shaking strength, and
enters the shaking time. A shaking time of 0s indicates no shaking. The maximum
shaking time is 999 seconds.
The Up and Down arrow key moves the cursor to the desired parameters. The Right
arrow key toggles between Yes/No (Select/Deselect) shaking or selects strength:
Low/Middle/High. The Numerical keys are used to enter the shaking time. The Enter
key seals the selection and returns the user to the previous screen.
c. Set Read Mode
Read Mode
In this screen, the user chooses Fast or Step reading and also chooses Normal or
Evaluation mode.
The “[ ]”indicates the current selection. The Up and Down arrow key moves the cursor
to the desired parameters. The Right arrow key chooses Fast/Step speed or chooses
Normal/Eval mode. The Enter key seals the selection and returns to the previous screen.
The followings are the description of each read mode parameter.
Reading speed
Fast reading: 6 sec/single wavelength reading, 10 sec/dual wavelength
reading
Step reading: 15 sec/single wavelength reading, 30 sec/dual wavelength
reading
Reading mode
Normal mode: Plate reading once
Evaluation mode: Four plate readings per one of measurement are performed and
the average data of their plate readings is generated as a plate
data.
Photometric: Dual
Measurement: 400 nm
Reference: 655 nm
Set Shake parameters
Shake:
Yes
Speed: Mid
Time: 199 sec(0-999)
» ■Reading speed
[Fast ] Step
•Read mode
[Normal] Eval
» ■Set Photo mode
•Set shaking
•Set read mode
Photometric: Single
Measurement: 400 nm