Bio-Rad Gel Doc™ EZ System - Manual (Page 87)

Criterion Stain Free TM System
73
Glossary
The following glossary defines words used in this Criterion Stain Free System
manual.
The equations used to calculate regression methods are also in this appendix,
see “Regression Method Calculations” (page 75).
2-Mercaptoethanol (BME):
A protein denaturant which breaks
disulfide bonds.
CCD:
(Charge-Coupled Device) A light-sensitive silicon chip used as a
photodetector in digital camera systems.
Charge-to-Mass Ratio:
The value measured by electrophoresis.
Colormaps:
Different color representations of a gel image; Stain Free
provides Gray, Stain Free, Coomassie, False Color, Spectrum, Gold-Silver,
Pseudo
Dithiotreitol (DTT):
A reducing agent
Electrophoresis:
A technique for separating molecules based on the
differential movement of charged particles through a matrix when subjected to
an electric field.
EtOH:
Ethanol.
Example precision:
The number of decimal places chosen for a
measurement.
Flat Fielding:
An average intensity computation which compensates
for non-uniformities generated by an instrument.
histogram:
A graphed representation of the brightness, or gray value, of
an image.
MeOH:
Methanol.
Native-PAGE:
Polyacrylamide gel electrophoresis performed without
the use of SDS.