Bio-Rad Gel Doc™ EZ System - Manual (Page 30)

Criterion Stain Free TM System | Criterion Stain Free Gels
16
Gel Specifications
Setup and Basic Operation
Setting Up and Running Criterion Gels
1. Each Criterion gel is packaged individually in a plastic storage tray.
Remove the cover by gently pulling the square corner tab up and
diagonally across the package. Remove the gel from the package.
2. Remove the comb and gently rinse the wells with double distilled H
2
O
or running buffer.
3. Remove the tape from the bottom of the cassette by pulling the tab
across the gel.
4. Insert the Criterion gel into one of the
slots in the Criterion cell tank. Ensure that
each integral buffer chamber faces the
center of the cell.
5. Fill each integral buffer chamber with
60 ml running buffer.
6. Load samples using a Hamilton syringe
or a pipet with gel loading tips. A sample
loading guide can be placed on the outer
edge of the cassette, to aid in aligning pipet tips with the wells. This is
especially useful with multichannel pipets.
Gel material Polyacrylamide
Gel dimensions 8.7 x 13.3 cm
Gel thickness 1.0 mm
Resolving gel height 6.5 cm
Cassette dimensions 10.6 x 15.0 cm
Cassette material Styrene copolymer
Comb material Polycarbonate
Storage tray material PET
Upper running buffer volume 60 ml
Lower running buffer volume 800 ml
Storage conditions Store flat at 4
°C; DO NOT FREEZE