Bio-Rad Gel Doc™ EZ System - Manual (Page 31)

Setup and Basic Operation
17
7. Fill each half of the lower buffer tank with 400 ml of running buffer to the
marked fill line.
8. Place the lid on the tank, aligning the color-coded banana plugs and
jacks. See “Running Conditions” (page 21) for power conditions.
Opening Criterion Cassettes and Removing
the Gel
1. After electrophoresis is complete, turn off the power supply and
disconnect the electrical leads.
2. Remove the lid from the tank, and remove the Criterion gel from the cell.
Pour off and discard the upper running buffer.
3. Invert the cassette and place the
integral buffer chamber over the
cassette-opening tool built into the
Criterion cell lid.
4. Firmly press down on the cassette to
crack the cassette welds on both
sides of the cassette. The cassette will
split open approximately 1/3 of the
way. Alternatively, the gel cassette can
be opened by sliding the tapered back
of the comb into the slits on either
side of the cassette.
5. Pull the two halves of the cassette
apart to completely expose the gel.
6. Carefully lift the gel from the cassette.
Placing the Gel on the
Sample Tray
Place the gel onto the sample tray
immediately after removal from the Criterion
cassette.
1. Place a few milliliters of water onto the sample tray, so that the gel can
slide easily. Don’t use so much water that it may spill inside the
instrument.
2. Place the gel on the sample tray, taking care to square the gel edges
and eliminate any bubbles which can distort the gel image.