Step 4. analyze molecular weight, Molecular weight standards – Bio-Rad Gel Doc™ EZ System User Manual
Page 52

Criterion Stain Free
TM
System | Designing Protocols
38
S
TEP
4. A
NALYZE
M
OLECULAR
W
EIGHT
If you want to estimate the molecular weight of the bands in your gel, use this
step to enter the standards you are using, and also to specify the lane(s) in
which the standards are placed.
You analyze molecular weight automatically by clicking Protocol Step
4. “Analyze Molecular Weight” shown above. If this box is checked, your
standards will be labeled with their molecular weights, for easy comparison
with your test samples.
M
OLECULAR
W
EIGHT
S
TANDARDS
Determining molecular weight depends upon the selection of the proper
standards. Four are available from Bio-Rad. See “Protein Standards” on
page 24 for catalog numbers.
•
Bio-Rad Precision Plus Protein
•
Bio-Rad Broad Range
•
Bio-Rad Low Range
•
Bio-Rad High Range
NOTE: Colored (or pre-stained) standards cannot be used with
this system, because they will not be detected or seen.