Eppendorf AF2200 Plate Reader User Manual
Page 76
Evaluation procedure
Eppendorf
®
PlateReader AF2200
English (EN)
76
Average raw data
Average value of control replicates / blank replicates / sample replicates in RFU
SD raw data
Standard deviation of control replicates / blank replicates / sample replicates in RFU
SD [%] raw data
Standard deviation of control replicates / blank replicates / sample replicates in %
Average blanked
Average value blanked = Average value of control replicates / sample replicates – average value of blank
replicates in RFU
SD blanked
Calculation of blanked standard deviation
% Gauss
Calculation of Gauss error.
% of control CellTiter-Blue Assay
Calculation of sample viability:
average viability of control replicates – average viability of blank replicates = 100%
average viability of sample replicates - average viability of blanks replicates = x%
% of control Apo-ONE Caspase-3/7 Assay
Calculation of caspase activity:
average caspase activity of control replicates – average caspase activity of blank replicates = 100%
average caspase activity of sample replicates - average caspase activity of blank replicates = x%
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