Eppendorf AF2200 Plate Reader - Manual (Page 49)

Defining measurements
Eppendorf ® PlateReader AF2200
English (EN)
49
5.5 Indenting and Releasing Program Elements
The decision to indent or release a program element will modify the workflow of the instrument during
measurements.
The actions of all program elements with the same indentation are performed sequentially. The only
dependence between these program elements is that the next action starts directly after the previous action
is finished.
A program element that is indented more than the previous program element shows dependence between
the two program elements. This means the parameters defined in the first program element are also active
for the second (indented) program element.
The following is an example of how to define a Multimode kinetic with two Absorbance measurements. The
example shows that the two Absorbance program elements depend on the Kinetic Cycle program element,
which depends on the Pattern program element, which depends on the Plate program element.
 Define the parameters for an example as follows:
• Plate: 96 well plate, e.g. Eppendorf 96 Flat Clear UV-VIS
• Kinetik Cycle/Number of cycles: 50
• Absorbance/Wavelength: 260 nm
• Number of reads: 25
• Measurement Name: 260
• Second Absorbance/Wavelength: 280 nm
• Number of reads: 25
• Measurement Name: 280