Eppendorf AF2200 Plate Reader - Manual (Page 19)

Overview
Eppendorf ® PlateReader AF2200
English (EN)
19
Multiple Reads per Well
The software allows the user to define multiple reads per well (MRW) in Absorbance, Fluorescence top and
Fluorescence bottom mode.
The MRW feature is not available for well wise measurements.
The Reference wavelength on the absorbance program element is not selectable in combination with
multiple reads per well .
The multiple reads per well function can be activated on an absorbance or fluorescence intensity program
element by selecting the Multiple reads per well check box.
The multiple reads per well function is available for plate formats with up to 384 wells.
4.2.3 Actions
Temperature
1. Select the Temperature program element to enter a certain target temperature.
2. Select On to enter a target temperature value.
3. Click on the link Wait until temperature is reached to define the Minimum and/or Maximum temperature
values. The heating of the instrument starts when clicking the Start button.
4. For pre-heating the instrument, select Heating in the Instrument menu and click the On button.
The measurement only starts if the current instrument temperature is within the specified range (see
Miscellaneous on p. 21).
More details on defining parameters for multiple reads per well, are available in the Operating
Manual of the Eppendorf PlateReader AF2200.