Eppendorf AF2200 Plate Reader - Manual (Page 18)

Overview
Eppendorf
® PlateReader AF2200
English (EN)
18
Gain The gain is an amplification factor for the photomultiplier tube (PMT)
and may be set by selecting one of the following modes:
Manual gain : user-defined gain value (valid range: 1-255)
Optimal gain : calculated automatically by the instrument according
to the highest signal within the selected well range in order to avoid
OVER. Optimal gain determination is performed in a
pre-measurement. It is recommended to use the optimal gain
function for all applications that produce results with unknown RFU
values.
Calculated from well : determines the optimal gain for the selected
well. The resulting gain value is applied to all other wells within the
selected well range.
Extended dynamic range : The extended dynamic range option is an
automatic gain function that serves to optimally adjust the gain
setting for both very high and very low signals on a microplate within
one single measurement. By selecting "extended dynamic range",
the measurement is done in two consecutive parts, one with a high
and one with a low gain. The results of both measurements are
automatically correlated and displayed within one single data set.
For measurements in combination with the Extended dynamic range function, a very high gain
value in the first measurement can result in OVER values in the second measurement. When
only very high and very low signal intensities are detected within the measured well range, the
new gain value is calculated on the basis of the very low values, in certain cases this can lead
to OVER for the entire second measurement.
Integration/Lag time Integration time : duration of signal recording per well (valid range:
20-2000 μs).
Lag time : time between flash and the start of signal integration.
While lag time is an optional function, the integration time is a
mandatory parameter for defining the duration of signal recording.
The default values for standard fluorescence intensity measurements
are 0 μs lag time and 20 μs integration time.