Eppendorf AF2200 Plate Reader - Manual (Page 73)

73
Troubleshooting
Eppendorf ® PlateReader AF2200
English (EN)
7 Troubleshooting
7.1 General Troubleshooting
If you don’t meet your expected measurement results please check the following:
• The volumes of all measured wells are homogeneous. Uniformity of pathlength [d] is essential for
reliable measurements.
• The homogeneity of your samples is given:
The bottom of the wells must be entirely covered with fluid.
Precipitation and irregular colour product distribution have to be avoided.
Buffers which have massive meniscus effects shouldn’t be used.
7.2 Troubleshooting Absorbance mode
Identical volumes of different fluid types and buffers may have different pathlengths.
Warning / error Consequences Remedy
Sample data out of
min-max-range of standard values Corresponding sample results are
marked red in the results table. Dilute sample or modify standard
concentrations and remeasure.
Correlation coefficient < 
user input Sample results are not calculated. Check input of standard
concentrations and remeasure.
Check concentrations of standard
solutions and remeasure
Reduce your required value of R
2
in the User Settings Dialog of the
software and remeasure.
At least one sample is out of
measurement range. Raw data of the samples are
displayed as
OVER , sample results
are not calculated.
Dilute sample and remeasure.
At least one standard is out of
measurement range. Raw data are displayed as OVER ,
standard curve is not calculated.
Dilute standard solution and
remeasure.
d