4 defining kinetic measurements, Defining kinetic measurements – Eppendorf AF2200 Plate Reader User Manual

Page 47

Defining measurements

Eppendorf

PlateReader AF2200

English (EN)

5.4

Defining Kinetic Measurements

The following example describes a kinetic measurement of a 96 well plate.

 Select the 96 well plate (e.g. Eppendorf 96 Flat Clear UV-VIS) from the

Plate definition

drop-down list,

and select all wells in the

Pattern

program element.

 Double-click the Kinetic Cycle program element and define as follows:

•

Cycles/Number of cycles: 50

•

Kinetic Interval (intervals between measurements): Select

Use kinetic interval

and enter: 2 minutes

30 seconds.

 Double-click the

Absorbance

program element and define as follows:

•

Wavelength/Measurement: 462 nm

•

Read/Number of reads: 25