Eppendorf AF2200 Plate Reader - Manual (Page 74)

Troubleshooting
Eppendorf
® PlateReader AF2200
English (EN)
74
7.3 Troubleshooting Fluorescence mode
7.4 Save method scripts
Predefined methods, with the exception of Nucleic acid quantification (UV 260 nm microvolume), may be
modified by the user and saved under another name (Save-as functionality) in the directory:
• Win XP: C:\ Documents and Settings\All Users\Documents\Eppendorf\Methods
• Win 7: C:\Users\Public\Documents\Eppendorf\Methods
These methods can be loaded for further measurements by selecting Open from method from the File menu.
7.5 Save method results
After performing a measurement using a predefined method, the results are automatically saved in the
following directory:
• Win XP: C:\ Documents and Settings\All Users\Documents\Eppendorf\Results
• Win 7: C:\Users\Public\Documents\Eppendorf\Results
Warning / error Consequences Remedy
Sample data out of
min-max-range of standard
values. Corresponding sample results are
marked red in the results table. Dilute sample or modify standard
concentrations and remeasure.
Correlation coefficient of standard
curve < user input. Sample results are not calculated. Check input of standard
concentrations and remeasure.
Check concentrations of standard
solutions and remeasure
Reduce your required value of R
2
in the User Settings Dialog of the
software and remeasure.
At least one sample is out of
measurement range. Raw data of the samples are
displayed as
OVER , sample results
are not calculated.
Check gain settings and
remeasure.
Dilute sample and remeasure.
At least one sample is out of
measurement range. Raw data are displayed as OVER ,
standard curve is not calculated.
Check gain settings and
remeasure.
Dilute standard and remeasure.
Calculation Error Standard curve is not calculated Define standard concentrations in
the method stripe of the software
and remeasure.