Eppendorf AF2200 Plate Reader - Manual (Page 56)

Predefined methods
Eppendorf
® PlateReader AF2200
English (EN)
56
Plate layout
Abb. 6-2: Method strip: Nucleic acid quantification (UV 260 nm with factor)
Fig. 6-2: Method strip: Nucleic acid quantification (UV 260 nm with factor)
The selected number of blanks and samples are shown in the plate view on the right side of the method
strip.
6.2.3 Nucleic acid quantification (UV 260 nm with standards)
Introduction
The most common technique for determining the concentration of a nucleic acid sample is based on
measuring the absorbance at 260 nm. By using two or more nucleic acid standards of known
concentrations, the nucleic acid concentrations of unknown samples can be obtained.
Default measurement parameters
• Measurement wavelength: 260 (5) nm
• Number of flashes: 25
• Settle time: 0 ms
• Plate definition: Eppendorf Microplate UV-VIS, 96/F or comparable plates
The method is restricted to 96-well UV transparent microplates.