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2 defining endpoint measurements, Defining endpoint measurements – Eppendorf AF2200 Plate Reader User Manual

Page 42

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Defining measurements
Eppendorf

®

PlateReader AF2200

English (EN)

42

5.2

Defining Endpoint Measurements

The following example describes an Absorbance Endpoint Measurement in all wells of a 96 well plate.

 Select a 96 well plate (e.g. Eppendorf 96 Flat Clear UV-VIS) from the

Plate definition

drop-down list. If

the

Pattern

program element is not visible, click the link

Pattern

. It is recommended to use the

Pattern

program element in every workflow, even if all wells are measured.

 Double-click the Absorbance program element from the Explorer bar, and define the Workflow as

follows:

Wavelength/Measurement: 462 nm

Read/Number of reads/flashes: 25 (per well)

Settle time (time between moving the plate and starting the measurement): 0 ms