Eppendorf AF2200 Plate Reader - Manual (Page 55)

Predefined methods
Eppendorf ® PlateReader AF2200
English (EN)
55
Default measurement parameters
• Measurement wavelength: 260 (5) nm
• Ratio wavelength (for purity check with 260/280 ratio): 280 (5) nm, optional
• Background wavelength (for correction of turbidity): 340 (10) nm, optional
• Number of flashes: 25
• Settle time: 0 ms
• Plate definition: Eppendorf Microplate UV-VIS, 96/F
User defined method parameters
• The measurement is performed at 260 nm with default measurement parameters.
• For a ratio measurement, select the Ratio wavelength 280 nm check box.
• For a background measurement, select the Background wavelength 340 nm check box.
• Ratio wavelength and background wavelength may also be combined.
• Define the number of blank replicates (possible number: 1–8).
• Define the number of samples (max. 95, depends on number of blank replicates).
• Select the type of sample under Sample type (possible selection: dsDNA, ssDNA, RNA).
• Select your sample volume under Sample volume (possible selection: 100; 150; 200; 250; 300 μL).
• Define the unit (possible selection: μg/mL or ng/μL).
• Check if entered parameters are correct.
• Required plate layout: Pipette in a vertical order, starting with blanks at position A1. Samples are
pipetted in subsequent wells.
• Press Start .
• For method results and data evaluation principle see chapter method results and evaluation procedure .
The method is restricted to Eppendorf Microplate UV-VIS, 96/F. The pathlengths are
determined for aquaeus buffer. For accurate results the meniscus of the sample has to be flat.
This method does not work for samples solved or diluted in detergent.