Eppendorf AF2200 Plate Reader - Manual (Page 16)

Overview
Eppendorf
® PlateReader AF2200
English (EN)
16
Well
Use the Well program element to perform measurements well by well. Without this program element, all
measurement steps are done plate-wise.
4.2.2 Mode
Absorbance
The Absorbance program element is used to perform absorbance measurements.
1. Enter or select the respective parameters:
Wavelength Specify a measurement wavelength.
The Reference wavelength may be selected to correct for flash
variations.
Two drop-down lists display the available measurement and
reference filter wavelengths, according to the inserted absorbance
filter slide. If the drop-down lists are empty, the absorbance filter
either has not been inserted into the reader or has not been defined.
Read Specify a certain Number of flashes and, if required, a Settle time
before the next measurement. The number of flashes is selectable
from 1 – 100.
Settle time : Enter a value to specify the time between the movement
of the plate carrier to the measurement position and the first flash.
Flashes In order to obtain a good measurement precision it is recommended
to perform absorbance measurements with the number of flashes
that is set as a default value for the respective instrument. On-the-fly
measurements with one flash per well are possible with all plate
types.
Measurement Enter a measurement name.