Eppendorf AF2200 Plate Reader - Manual (Page 17)

Overview
Eppendorf ® PlateReader AF2200
English (EN)
17
Fluorescence Intensity
The Fluorescence Intensity program element contains fields for the selection of excitation and emission
wavelength, top or bottom reading mode, integration and lag time, flash number and gain settings. A
checkbox for multiple reads per well gives access to additional function.
The following are the Fluorescence Intensity parameters:
Wavelength and Bandwidth Specify an Excitation and an Emission wavelength.
Two drop-down lists display the available measurement filter
wavelengths.
If the spin box of fixed values is empty, the excitation and emission
filters have not been inserted into the reader or have not been
defined.
The bandwidth is specific for every used filter and needs to be
defined together with the central filter wavelength.
Read Specify a certain Number of flashes and, if required, a Settle time
before the next measurement. The number of flashes is selectable
from 1 – 100.
Settle time : Enter a value to specify the time between the movement
of the plate carrier to the measurement position and the first flash.
Flashes In order to obtain a good measurement precision it is recommended
to perform fluorescence measurements with the number of flashes
that is set as a default value for the respective instrument. On-the-fly
measurements with one flash per well are possible with all plate
types.
Mode Select Top or Bottom.
Measurement Enter a measurement name