Eppendorf Mastercycler nexus - Manual (Page 78)

System management
Mastercycler
® nexus
English (EN)
78
Example
 Make the desired settings in the header (see Editing header settings on p. 52).
1 T = 94.0° 0:02:00
2 T = 94.0° 0:00:15
3 T = 60.0° 0:00:15
+0.0° +0:00
R = 3.0°/s
+0:0°/s
G =10.0°
4 T = 72.0° 0:00:30
5 GOTO 2 REP 29
6 T = 72.0° 0:02:00
7 HOLD 4.0° ENTER
For the Mastercycler nexus you need to enter the gradient spreads.
A gradient setting of G = 10.0° for the Mastercycler gradient corresponds to a gradient spread
of 20.0°C for the Mastercycler nexus gradient. Enter this value in the
Span field.