Eppendorf Mastercycler nexus - Manual (Page 58)

Programming
Mastercycler
® nexus
English (EN)
58
6.4 Program templates
The following table provides an overview of the program templates. The program templates can be used to
create new programs.
Program
template Description
none Empty template with standard header settings.
2-step PCR Standard PCR program with two temperatures per cycle and 30 cycles, one hold step
at the end.
3-step PCR Standard PCR program with three temperatures per cycle and 30 cycles, one hold
step at the end.
Cycle sequencing Standard program for cycle sequencing with ramp rates of 1 °C/s.
Fast PCR 3-step PCR with short hold times and temperature mode fast
Gradient PCR PCR program with a temperature gradient in the annealing step of each cycle.
Hot start PCR
manual PCR program with thermoblock preheating at 95 °C. Start the following PCR run
using the start key.
Hot start PCR 10 min enzyme activation at 95 °C and 3-step PCR.
Incubation IsoTherm incubation.
Large volume PCR 3-step PCR in safe temperature mode.
Long range PCR 3-step PCR with 10 min elongation and a time interval of +10 s per cycle.
Low volume PCR 3-step PCR in fast temperature mode.
Mastercycler 533x 3-step PCR with simulation of the temperature control behavior of the
Mastercycler gradient 5331.
Nested cycles Program in which 3 cycles, with 3 temperatures each, are summarized in a
9-StepCycle, e.g., for TAIL PCR.
Reduced Ramping 3-step PCR with ramp rates of 1 °C/s.
Reverse
Transcription Incubation for reverse transcription and enzyme activation.
RT-PCR Program for 1-step RT-PCR with series incubation for reverse transcription.
Touchdown PCR PCR program with falling annealing temperature. During the first 16 cycles, the
annealing temperature will be decreased by 1 °C per cycle.