Eppendorf Mastercycler nexus - Manual (Page 33)

Operation
Mastercycler ® nexus
English (EN)
33
5 Operation
5.1 Initial steps
Checking the correct installation
Before the Mastercycler nexus is commissioned for the first time, ensure that the following requirements
are met:
• The device is correctly connected.
• The device is free of damage.
• Free circulation of air around the ventilation slots at the rear of the device and at the fan behind the front
feet has been guaranteed.
5.1.1 Using the flexlid heated lid
The Mastercycler nexus is equipped with a flexlid heated lid. The flexlid heated lid automatically adjusts to
the height of the used PCR tubes or PCR plates. It can be operated with one hand. Manual adjustment to
the tube height, or adjusting the contact pressure to the tube lids, is not required.
The heated lid ensures uniform contact between the tubes and the thermoblock, and secures the tube lids
against leaks. The heating process prevents condensation from forming in the upper area of the tube during
the tempering of the reaction liquid.
5.1.1.1 Open heated lid
Open the heated lid in one go as follows:
 Flip the lid handle up over the heated lid and flip the heated lid up at the lid handle as far as it will go.
WARNING! Biohazard due to temperature control when heated lid is open.
If the heated lid is open, the lids of the reaction vessels may come off during temperature
control. This will release sample material.
 Tempering may only be carried out when the heated lid is closed.
CAUTION! Risk of burns on thermoblock, heated lid and reaction vessels.
The thermoblock, heated lid and reaction vessels reach temperatures of more than 50 °C very
quickly.
During gradient operation, the temperature of the heated lid and thermoblock is higher on the
right side than on the left side.
 Wait until the temperature of the thermoblock, heated lid and reaction vessels is below 30
°C.
 Then the heated lid may be opened.