Eppendorf Mastercycler nexus - Manual (Page 61)

PCR run
Mastercycler ® nexus
English (EN)
61
Observe the following notes when loading the thermoblock with tubes
 Place the tubes or strips into the center of the thermoblock and ensure that they are symmetrically
positioned. This is applicable for individual tubes, ube strips and segments of separable PCR plates.
 Load the 96-well thermoblock with at least 5 tubes. If you have fewer reaction batches, also add empty
tubes so the thermoblock is loaded with at least 5 tubes.
 Load the 64+32-well thermoblock with at least 2 tubes. If you have fewer reaction batches, also add
empty tubes so the thermoblock is loaded with at least 2 tubes.
7.2 Starting a program
Prerequisites
• Thermoblock loaded (see p. 59)
• Heated lid closed (see p. 34)
1. Mark the program in the navigation tree with the arrow keys or open it in the editor.
2. Press the start key.
If multiple cyclers are available, the Cycler Selection window appears.
3. Mark the desired cycler.
4. Press the OK softkey.
The program is started and the status view appears.
Very soft PCR tubes may become deformed at high temperatures. To prevent deformations:
• Increase the number of empty sample tubes in the thermoblock.
• Reduce the temperature of the heated lid (see p. 52).
• Use original Eppendorf PCR consumables. Eppendorf PCR consumables are optimally
suited for the device.
If the PIN function is activated, you can only start programs that are saved below your user
node (see p. 67).