Eppendorf Mastercycler nexus - Manual (Page 52)

Programming
Mastercycler
® nexus
English (EN)
52
6.2.2 Editing header settings
Lidtemp. Heated lid temperature (37°C to 110°C). The set lid temperature is maintained
constant during the program run.
If values below 37°C are entered, the heated lid remains switched off.
TSP heated lid Preheat heated lid
• Switch TSP on and off.
If the TSP heated lid function is activated, the heated lid is heated up to the set
temperature upon program start while the block temperature is maintained
constant at 20°C. The program run starts once the heated lid temperature has
been reached.
switch off lid at low
blocktemperature Lid heater with automatic switch-off
• Activated (default setting): the lid heater switches off if program steps have block
temperatures of < 15°C.
• Deactivated: the lid heater switches off after the end of the program. If the last
command of the program is a hold step, the heated lid remains switched on until
you press the Enter key.
Note: This option is useful if you have inserted a hold step as the last program step in
order to automatically cool the samples after the PCR.
Temp. mode Block temperature control mode
• fast :
Use this setting for Fast PCR, for low reaction volumes (< 20 μL) and for templates
with a low G/C content. With this setting you achieve the shortest program run
times.
• standard : Block temperature control mode for standard applications.
Use this setting for sample volumes between 20 μL and 50 μL or if the fast setting
only results in a weak amplification. The program run times are slightly longer
than with the
fast setting.
• safe : Block temperature control mode for complex templates and large reaction
volumes.
Use this setting for sample volumes of 50 μL or more or to amplify templates with
a high G+C content. The program run times are slightly longer than with the
standard setting.
Simulate
Mastercycler
gradient Simulate the temperature control behavior of the Mastercycler or Mastercycler
gradient. This enables you to transfer programs from these cycler models to the
Mastercycler nexus without making any adjustments (see Transferring programs from
an older Mastercycler model on p. 77).
If this option is deactivated, programs created for the Mastercycler will need to
adapted for the Mastercycler nexus because of different temperature behavior. This
may influence the PCR results.