Time acquisition experiments – Ocean Optics S1024DW Install User Manual

Page 59

Time Acquisition Experiments

Time acquisition experiments track processes, perform kinetic analyses, and monitor spectral events as a
function of time. (See the

OOIBase32 Spectrometer Operating Software Manual

for details.)You can

collect as a function of time spectral data from up to 6 single wavelengths (designated as Channels A
through F) and up to two mathematical combinations of these wavelengths (designated as Combinations 1
and 2). You can acquire data in any mode. To perform a time series experiment, follow these steps:

1. While in scope mode, store reference and dark spectra, if desired, and choose a view mode, such as

absorbance.

2. Access the Time Acquisition Channel

Configuration dialog box by selecting Time
Acquisition | Configure | Configure Time
Channels from the menu.

•

Select Enabled to set the time acquisition
calculation for the wavelength. Without
selecting Enabled, the time acquisition
process will not calculate data.

•

Enable Plotted to see a real time graph of
the acquired data in a spectral window.

•

Select a Spectrometer Channel for your
time acquisition process.

•

In the Wavelength (nm) box, specify the
analysis wavelength. In the Bandwidth
(pixels) box, specify the number of pixels
averaged around the analysis wavelength.

•

Select a multiplicative Factor to apply to the data before it is plotted or stored. Select an additive
constant or Offset to apply to the data after the factor is applied and before the data is plotted or
stored. The equation for the Factor and Offset functions is: Results = (Factor x Data) + Offset.

3. If desired, configure a time acquisition process for the second single wavelength by selecting the

Channel B page. To configure a time acquisition process for the third, fourth, fifth, and sixth single
wavelengths, select the Channel C, Channel D, Channel E, and Channel F pages, respectively, and
set the necessary parameters.

4. If desired, configure a time acquisition process for a combination of two time channels by selecting

Combination 1.

•

Select Enabled to set the time acquisition calculation for the wavelength.

•

Enable Plotted to see a real-time graph of the acquired data in a spectral window.

•

Specify Time Channel A through F for the First Channel.

•

Select the mathematical Operation to produce the data for Combination 1.

•

Specify Time Channel A through F for the Second Channel.

•

5. If desired, configure a time acquisition process for the Combination 2 page. It is almost identical to

the Combination 1 page except that you have the option of choosing Combination 1 for your First
Channel or Second Channel.

6. Click the OK button for the Time Acquisition Channel Configuration dialog box.