Ocean Optics S1024DW Install - Manual (Page 38)

35
Assessing if the LPC is Free of Particles
Fluids need to be relatively particle-free. Particles larger than 20
µ
m can be trapped inside the tubing and
can then block or scatter a significant amount of light. To rid the LPC of particles, follow these steps:
1. Pump the sample fluid through the LPC.
2. While in Scope Mode, save a dark spectrum with the light source off and a reference spectrum with the
light source on.
3. Continue to pump the sample fluid and switch to the Absorbance Mode. Ideally, you should see a
spectrally flat line. Particle effects manifest themselves as an exponentially decreasing curve from shorter
to longer wavelengths. The length of time that you pump the sample and the magnitude of the absorbance
peak depends upon the time required and the minimum detectable absorbance value for your specific
analyses. Pre-filtering of the sample may be required to eliminate this exponentially decreasing
absorbance spectrum if it is significant to your analyses.
Specifications
Path lengths:
1-meter, 5-meter and 10-meter options (standard);
custom lengths also available
Tubing:
•
Teflon

Amorphous Fluoropolymer 2400
•
~560
µ
m inner diameter, ~800
µ
m outer diameter
Refractive index: 1.29
Internal volume: 250
µ
l/meter
Chemical resistance:
tubing can be altered by perfluorinated solvents,
FREON

113, and Perclene

Recommended optical fibers for coupling to
spectrometers, light sources:
•
400
µ
m illumination fiber (UV-VIS)
•
200
µ
m or 400
µ
m read fiber (UV-VIS)
Plumbing fittings: standard ¼” x 28 chromatography fittings