Ocean Optics S1024DW Install - Manual (Page 29)

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Sampling Chambers: CUV-ALL 4-way Cuvette Holder
The CUV-ALL 4-W AY C UVETTE H OLDER for 1-cm path length cuvettes has four collimating lenses that
couple to optical fibers, light sources, and spectrometers to measure absorbance, fluorescence, scattering, or
any combination of these optical phenomena.
Parts Included
•
CUV-ALL assembly for holding 1-cm cuvettes
•
Black Cover for eliminating ambient light
•
Two Barbed Fittings for connecting a temperature stabilizing water source
•
Screwdriver for adjusting the fit of the cuvette
•
Allen wrench for adjusting the collimating lenses
Operation
Attaching the Fibers for Absorbance Measurements
1. For UV measurements, attach one end of a SMA-terminated optical fiber to one of the collimating
lenses with a red lens cap. Attach the other end of this fiber to a UV light source. Attach another SMA-
terminated optical fiber to the collimating lens directly opposite of the first fiber. It too has a red lens
cover. Attach the other end of this fiber to the spectrometer.
2. For VIS measurements, attach one end of a SMA-terminated optical fiber to one of the collimating
lenses with a black lens cover. Attach the other end of this fiber to a light source. Attach another SMA-
terminated optical fiber to the collimating lens directly opposite of the first fiber. It too will have a
black lens cover. Attach the other end of this fiber to the spectrometer.
Attaching the Fibers for Fluorescence Measurements
1. Attach one end of a SMA-terminated optical fiber to one of the collimating lenses. Attach the other end
of this fiber to a light or excitation source. Typically, for fluorescence measurements, the illumination
fiber connects to a UV lens.
2. Attach another SMA-terminated optical fiber to the collimating lens to the immediate left or right of
the first fiber. The two collimators must be positioned at 90° for fluorescence measurements. Attach
the other end of this fiber to the spectrometer. Typically, for fluorescence measurements, the read fiber
connects to a VIS lens.
Using the Fiber Supports
1. Snap the clamps around the fibers after the fibers are screwed into the light source and spectrometer.
2. Lift the clamps until they support the fibers.
3. Unsnap the clamps to remove the fibers.
Adjusting the Fit of the Cuvette
The CUV-ALL is preset for use with a 1-cm quartz cuvette. When properly adjusted, the cuvette should fit
snugly in the holder.
1. Locate the two ball plunger screws. They are under two of the four collimating lenses.
2. Remove the fiber clamps for easier access to the screws.
3. Use the provided screwdriver to loosen the two ball plunger screws until the ball end of the screws is
just visible in the cell holder.
4. Insert your cuvette into the holder.
5. Tighten the ball plunger screws until the ball contacts the cuvette and starts to compress. Do not
over-tighten.