Fragment size (in bp) – Bio-Rad Experion DNA Analysis Kits User Manual
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Fig . A .1 . Electropherogram generated by an Experion DNA 1K analysis
.
Peaks identified by Experion software are numbered,
and the relative positions of the lower and upper markers are indicated by asterisks (*).
Fig . A .2 . Virtual gel generated by an Experion DNA 1K analysis . Shown are
separations of the DNA ladder (L) and 11 samples on a single chip.
Upper marker
Lower marker
Once separation is complete, the software subtracts background noise, removes spikes, identifies
and integrates peaks, and assigns their sizes and concentrations. The results of data analysis arealso
tabulated and presented in a Results tab (Figure A.3). The types of data available include the following:
n
Fragment size (in bp)
n
Concentration: relative concentration under Conc . (ng/µl) and Molarity (nmol/l)
n
% Total sample (percentage determination)
Experion DNA 1K and DNA 12K Analysis Kits