Bio-Rad Experion DNA Analysis Kits - Manual (Page 34)

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5.1 DNA Quantitation Methods
Experion ™ software enables DNA quantitation by several approaches: percentage determination
and concentration determination (yielding either ng/ul or molarity) (Table 5.1). These calculations are
performed automatically for each peak identified by Experion software and presented in the Results tab
(Figure 5.1).
Table 5.1. DNA quantitation methods used by Experion software.
Quantitation Method Output Description
Percentage Determination % Total Percentage of total DNA
Concentration Determination ng/µl Relative concentration (against upper marker)
nmol/L
Fig . 5 .1 . Results table showing quantitation data for peaks in a sample . Experion software automatically
performs % total and relative concentration determinations for each peak identified in every sample.
This change affects a selected sample. To exclude multiple peaks from multiple samples, use
the Peak Find Settings options in the Settings tab (see Section 6.3, Excluding a Peak From
Analysis and 6.4, Changing Peak Finding Parameters) and adjust the peak detection criteria.
5.2 Performing Percentage Determination
Experion software automatically calculates a percentage of total DNA (% total) value for each detected
peak and lists the results in the % Total column of the Results tab (Figure 5.1). Double-click on a peak
in the electropherogram (or right-click on a band in the virtual gel) to highlight the data for that peak in
the Results tab.
To exclude a peak from the % total calculation, right-click on the peak (in either the single-well
electropherogram view or Results table) and select Exclude Peak in the context menu. The new
calculations for the remaining peaks appear in the Results tab.
Experion Automated Electrophoresis System