Bio-Rad Experion DNA Analysis Kits - Manual (Page 38)

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6.1 Designating and Searching for Specific DNA Fragments
1. Designate and search for specific DNA fragments in your samples:
a. In the DNA Search Criteria tab, click Add DNA Name , or in the electropherogram, right-
click on a peak and select Add to Search List. The DNA Search List Criteria tab opens and
contains the size and tolerance range for that entry.
b. Click in the field under DNA Name and enter the fragment name.
c. Click in the field under Size (bp) and enter the size of the fragment in bp.
d. Click in the field under the ±bp mass column to enter the new value for the tolerance in bp.
2. A list of all the peaks that fit the criteria appears in the DNA Search Result tab. Separate tables
appear for each fragment designated in the search criteria list.
3. To delete designated fragments (“DNA names”) from a search, click Delete DNA Name in the
toolbar of the DNA Search Criteria tab.
6.2 Manually Setting a Marker
To designate a peak as a marker (and change the alignment of a sample), right-click on the peak in
either the electropherogram (single-well view) or the Results table and select Manually Set Lower
Marker or Manually Set Upper Marker to define the peak as the new lower or upper marker. Do this if
no or incorrect peaks have been selected as the lower or upper marker.
Pink triangles in the virtual gel indicate markers. The markers should be aligned across all lanes.
6.3 Excluding a Peak from Analysis
To exclude one or more peaks from analysis of a single sample, right-click on the peak in either the
electropherogram (single-well view) or Results table and select Exclude Peak from the context menu.
This change affects only a selected sample.
To exclude multiple peaks from multiple samples, open the Settings tab and use the Peak Find
Settings options in the All Wells Settings dialog (see Section 6.4, Changing Peak Finding Parameters)
to adjust the criteria by which peaks are detected.
Excluded peaks are not included in total DNA calculations. Therefore, excluding a peak from analysis
affects percentage determination (% total calculations) for the other peaks in a sample.
Experion Automated Electrophoresis System