Bio-Rad Experion DNA Analysis Kits - Manual (Page 47)

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Error Probable Cause Recommended Action
Upper or lower marker is
identified incorrectly Sometimes Experion software selects incorrect
peak to correspond to the upper or lower
marker; such an error affects alignment and
sizing Manually select the upper and lower markers by following
the instructions in Section 6.2
Virtual gel does not
resemble an agarose gel
separation of the same
samples The gel view is a direct interpretation of
the electropherogram data. By default, the
fluorescence intensity of the bands in each
lane is scaled to the intensity/peak height
of the highest peak in that sample (scale to
local). Consequently, each gel lane may have a
different scale of fluorescence intensity Adjust scaling of the virtual gel to Scale To Global; this also
adjusts the scale on all the electropherograms.
Adjust the intensity of the virtual gel using the sliding cursor
Baseline is fluctuating (for
example into very negative
and positive values) Air bubbles are interfering with electrical
contact in one or more of the wells Stop the run, remove the chip, and remove and replace
solution in affected wells
Chip is not completely primed Prime and load a new chip
Samples are too concentrated; DNA levels are
out of the range of the assay Dilute the samples and repeat the analysis
7.3 Contacting Technical Support
If you are having problems with a run and would like the Bio-Rad Technical Support team to review your
data, please submit the following files to LSG_TechServ_US@bio-rad .com for analysis:
n
Run file (C:\Program Files\Bio-Rad Laboratories\Experion Software\Data\
name>\.bdf)
n
Run log (C:\Program Files\Bio-Rad Laboratories\Experion Software\Data\
name>\.log)
n
Packet file (C:\Program Files\Bio-Rad Laboratories\Experion Software\Data\
name>\Packet\.txt)
n
System log (C:\Program Files\Bio-Rad Laboratories\Experion Software\Backup\Version X\
Experion_System.log or .txt)
n
Lot numbers of the DNA chips and the Experion DNA analysis kit
To export the run file, log file, and packet file, select the run(s) in the tree view and click Export Selected
Runs. The software exports the files into folders with the corresponding project name(s).
Experion DNA 1K and DNA 12K Analysis Kits