Bio-Rad PROTEAN® IEF Cell Accessories User Manual
Page 46
Catalog
Number
Product Description
165-4070
Forceps,
1 pair
165-4071
Electrode wicks, pre-cut, 4 mm x 20 mm,
500
165-4072
Cleaning brushes,
2
165-4080
Thermal Printer, with cable and power adaptor,
100 V
165-4082
Thermal Printer,
120 V
165-4085
Thermal Printer,
220 V
170-2412
Thermal Printer Paper,
10 rolls
163-2129
Mineral oil,
500 ml
ReadyStrip™ IPG Strips
163-2000
ReadyStrip IPG Strips pH 3-10, 7 cm,
12
163-2001
ReadyStrip IPG Strips pH 4-7, 7 cm,
12
163-2003
ReadyStrip IPG Strips pH 3-6, 7 cm,
12
163-2004
ReadyStrip IPG Strips pH 5-8, 7 cm,
12
163-2005
ReadyStrip IPG Strips pH 7-10, 7 cm,
12
163-2007
ReadyStrip IPG Strips pH 3-10, 17 cm,
12
163-2008
ReadyStrip IPG Strips pH 4-7, 17 cm,
12
163-2010
ReadyStrip IPG Strips pH 3-6, 17 cm,
12
163-2011
ReadyStrip IPG Strips pH 5-8, 17 cm,
12
163-2012
ReadyStrip IPG Strips pH 7-10, 17 cm,
12
Section 16
References
General 2D electrophoresis
1.
O'Farrell P. H. (1975) High resolution two-dimensional electrophoresis of proteins., J.Biol.Chem.
250, 4007–4021.
General solubilization
2.
Rabilloud, T. (1996) Solubilization of proteins for electrophoretic analyses. Electrophoresis, 17,
813–829.
3.
Link A.J.,Ed.(1999). "2-D Proteome Analysis Protocols." Methods in Molecular Biology, 112.
Humana Press, Totowa, NJ.
Thiourea /TBP
4.
Molloy M. P. et.al. (1998) Extraction of membrane proteins by differential solubilization for sep-
aration using two-dimensional gel electrophoresis. Electrophoresis, 19, 837–844.
5.
Herbert, B. R. et.al. (1998) Improved protein solubility in two-dimensional electrophoresis using
tributyl phosphine as reducing agent. Electrophoresis, 19, 845–851.
6.
Rabilloud T. (1998) Use of thiourea to increase the solubility of membrane proteins in two
dimensional electrophoresis. Electrophoresis, 19, 758–760.
7.
Ruegg, U. T. and Rudinger, J. (1977) Reductive cleavage of cystine disulfides with
tributylphosphine, Methods Enzymol., 47, 11–116.
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