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Bio-Rad PROTEAN® IEF Cell Accessories User Manual

Page 46

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Catalog
Number

Product Description

165-4070

Forceps,

1 pair

165-4071

Electrode wicks, pre-cut, 4 mm x 20 mm,

500

165-4072

Cleaning brushes,

2

165-4080

Thermal Printer, with cable and power adaptor,

100 V

165-4082

Thermal Printer,

120 V

165-4085

Thermal Printer,

220 V

170-2412

Thermal Printer Paper,

10 rolls

163-2129

Mineral oil,

500 ml

ReadyStrip™ IPG Strips

163-2000

ReadyStrip IPG Strips pH 3-10, 7 cm,

12

163-2001

ReadyStrip IPG Strips pH 4-7, 7 cm,

12

163-2003

ReadyStrip IPG Strips pH 3-6, 7 cm,

12

163-2004

ReadyStrip IPG Strips pH 5-8, 7 cm,

12

163-2005

ReadyStrip IPG Strips pH 7-10, 7 cm,

12

163-2007

ReadyStrip IPG Strips pH 3-10, 17 cm,

12

163-2008

ReadyStrip IPG Strips pH 4-7, 17 cm,

12

163-2010

ReadyStrip IPG Strips pH 3-6, 17 cm,

12

163-2011

ReadyStrip IPG Strips pH 5-8, 17 cm,

12

163-2012

ReadyStrip IPG Strips pH 7-10, 17 cm,

12

Section 16
References

General 2D electrophoresis

1.

O'Farrell P. H. (1975) High resolution two-dimensional electrophoresis of proteins., J.Biol.Chem.
250, 4007–4021.

General solubilization

2.

Rabilloud, T. (1996) Solubilization of proteins for electrophoretic analyses. Electrophoresis, 17,
813–829.

3.

Link A.J.,Ed.(1999). "2-D Proteome Analysis Protocols." Methods in Molecular Biology, 112.
Humana Press, Totowa, NJ.

Thiourea /TBP

4.

Molloy M. P. et.al. (1998) Extraction of membrane proteins by differential solubilization for sep-
aration using two-dimensional gel electrophoresis. Electrophoresis, 19, 837–844.

5.

Herbert, B. R. et.al. (1998) Improved protein solubility in two-dimensional electrophoresis using
tributyl phosphine as reducing agent. Electrophoresis, 19, 845–851.

6.

Rabilloud T. (1998) Use of thiourea to increase the solubility of membrane proteins in two
dimensional electrophoresis. Electrophoresis, 19, 758–760.

7.

Ruegg, U. T. and Rudinger, J. (1977) Reductive cleavage of cystine disulfides with
tributylphosphine, Methods Enzymol., 47, 11–116.

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