Bio-Rad PROTEAN® IEF Cell Accessories User Manual

Page 17

4.2 Preset Method Programming Instructions

1. Select the PRESET METHOD mode to program

a preset method.

2. Select one of the three voltage ramping

methods (slope). The difference between the
methods is the manner in which the voltage is
ramped to the maximum voltage (refer to Step
S2 in Figure 5).

3. Select the rehydration step (optional). No is the

default.

Select the gel size (7, 11, or 17 cm). Default is
07 cm. The gel size entered determines the
maximum voltage that is applied, 4000 V/7 cm,
8000 V/11 cm, and 10,000 V/17 cm.

Enter a focusing temperature between 10–25 °C.
20 °C is the default temperature.

Press the NEXT soft key to continue.

Note: Proceed to Step 4 if a rehydration step is
programmed. Proceed to Step 6 if a rehydration
step is not programmed.

Select the Active (50 V) or Passive rehydration
method. Passive rehydration is the default.

Enter a rehydration temperature between
10–25 °C. 20 °C is the default temperature.

Note: Maintain the rehydration and running
temperature above 18 °C if the rehydration
buffer contains urea.

5. Enter a time for the rehydration step up to 99:00

hours. The default value is 12 hours.

Select yes or no to insert a pause after the
rehydration step. NO pause is the default.

Press the NEXT key to continue.

Note: When a pause is programmed, the method will pause when the rehydration step is
complete to allow for the transfer of strips to a focusing tray, insertion of electrode wicks,
sample application, or the addition of mineral oil. If a pause is not programmed the method
will automatically continue with the first focusing step after rehydration is completed. Therefore,
be sure the strips are covered with mineral oil or similar overlay material during rehydration.

REHYDRATION

PRESET METHOD

STORED METHOD

NEW METHOD

SELECT VOLTAGE SLOPE:

RAPID

∆

LINEAR

∆

SLOW

∆

REHYDRATION: yes NO

GEL LENGTH 07

11 17

FOCUS TEMP: 20°

REHYDRATION

active@50V PASSIVE

ENTER TEMP: 20 °C

ENTER TIME:

12:00

INSERT PAUSE AFTER

REHYDRATION? yes NO