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1 new method programming diagram – Bio-Rad PROTEAN® IEF Cell Accessories User Manual

Page 24

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6.1 New Method Programming Diagram

21

"

Method Name"

SAVE METHOD? YES

no >

NEXT

>

REHYDRATION

>

PRESET METHOD

>

STORED METHOD

>

NEW METHOD

>

METHOD MEMORY FULL.

DELETE A STORED

METHOD? YES

no

>

NEXT

>

NAME: "

Method Name"

>

REHYDRATION: yes NO >

FOCUS TEMP: 20 °C >

NEXT

>

REHYDRATION STEP

active@50 V PASSIVE >

ENTER TEMP: 20 °C >

NEXT

>

ENTER TIME:

12:00

>

INSERT PAUSE AFTER

REHYDRATION? yes NO

>

NEXT

>

S 01

0 V >

SELECT VOLTAGE SLOPE:

RAPID

[

]

>

NEXT

>

METHOD "

Method Name"

INCOMPLETE STEP

PLEASE ENTER VOLTAGE.

or

(PLEASE ENTER TIME.)

NEXT

>

ENTER # OF GELS:

# >

µA LIMIT/GEL: 50

>

FOCUS TEMP:

20 °C >

PRESS

TO START

S 01

xxxxxV

HRS:MIN

vhours >

ENTER TIME:

00:00

>

NEXT

>

ADD

(EDIT) insert delete

>

ENTER STEP #

# >

NEXT

>

METHOD COMPLETE.

>

TO RUNNING

SCREENS

See Load stored method
screens for deleting a

method.

NEXT IF

NO

REHYDRATION

SELECTED

NEXT IF YES

NO

REHYDRATION

Voltage

Time

EXIT

NEXT

NEXT, if YES or NO