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Bio-Rad PROTEAN® IEF Cell Accessories User Manual

Page 30

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8.2 Program Status Screen Instructions

1. Start Run Screen

Enter the number of IPG strips to run.

Enter a current limit up to 99 µA.

Enter the focusing temperature (10–25 °C).

Press

to start the run.

2. Active/Passive Rehydration Display.

The actual and set temperatures are
displayed (actual/set).

Time is listed as elapsed time/programmed
time.

Press

to stop the rehydration step.

3. When cell cover is opened the method is

paused. Close the lid to resume the rehydration
step.

4. Rehydration Complete Display - This screen is

displayed when the rehydration step is complete
and a pause after rehydration step has been
programmed. In the pause mode the system
maintains the programmed temperature. At this
point IPG strips can be transferred to the focusing
trays if needed, electrode wicks can be inserted
and sample and/or mineral oil or similar overlay
material can be added to the individual chan-
nels.

Press

to start step 1 of the method.

5. S#/# (Current Step) Display

The actual voltage and the total current are
displayed.

The actual / set temperatures and the
current limit per gel are displayed.

The Total VH listed are cumulative but do
not include the rehydration step (active) or
the optional final hold step.

Time or vhours is listed as elapsed time
(vhours)/programmed time (vhours).

The Method Name is displayed. "Run in
progress" is scrolled during the run.

27

REHYDRATION COMPLETE.

PRESS

TO START

STEP #01 OF METHOD

** CELL COVER OPEN **

REHYDRATION PAUSED

CLOSE COVER TO PROCEED

ENTER # OF GELS:

1 >

µA LIMIT/GEL:

50 >

FOCUS TEMP:

20 °C >

PRESS

TO START

REHYDRATION: AT 50 V

TEMP: 20/20 °C

TIME: 00:00/00:00

METHOD WILL START (OR PAUSE)
WHEN REHYDRATION COMPLETES
(Scrolled)

S 01/#

XXV

XXµA

20/20 °C

XXµA/GEL

VH: 000000

00:00/XX:XX

NAME

RUN IN PROGRESS

(Scrolled message during
run)