Bio-Rad PROTEAN® IEF Cell Accessories User Manual

Page 25

6.2 New Method Programming Instructions

1. Select the NEW METHOD mode to program

a new method.

2. Use the adjacent soft-key to activate the

alphanumeric keypad and enter a program
name with up to 10 characters.

Select YES or No for a rehydration step
(optional). NO is the default.

Enter a focusing temperature between 10–25 °C.
20 °C is the default value.

Press the NEXT key to continue.

Note: If a rehydration step is programmed
proceed to Step 3. If a rehydration step is not
programmed proceed to Step 5.

3. Select the Active (50 V) or Passive rehydration

option. Passive rehydration is the default.

Enter a rehydration temperature between
10–25 °C. Press the NEXT key to continue

4. Enter a rehydration time between 1 minute and

99 hours.

Select yes to insert a pause after the rehydra-
tion step. Select no to proceed immediately with
the first focusing step.

Press the NEXT key to continue.

Note: A pause is needed for inserting electrode
wicks, adding mineral oil, adding sample, or
transferring the strips from the disposable tray
to the running tray.

Use the adjacent soft-key to activate the
alphanumeric keypad and enter the desired
voltage value (50–10,000 V).
Select the voltage ramping slope for the step.
Brackets [ ] will surround the selected option.

Note: Rapid [

] voltage ramping is limited

by the current. The Linear [

] voltage ramping

option increases voltage linearly with respect to

time. The Slow [

] voltage ramping option

increases voltage according to a delayed voltage

ramping algorithm.

Press the NEXT key to continue.

REHYDRATION

PRESET METHOD

STORED METHOD

NEW METHOD

NAME: "

METHOD NAME"

REHYDRATION: yes NO

FOCUS TEMP: 20 °C

REHYDRATION STEP

active@50 V

PASSIVE >

ENTER TEMP: 20 °C

ENTER TIME:

12:00

INSERT PAUSE AFTER

REHYDRATION? YES

S 01

0 V >

SELECT VOLTAGE SLOPE:

RAPID [

]