Bio-Rad PROTEAN® IEF Cell Accessories - Manual (Page 39)

If a focusing tray is used for the rehydration step, carefully insert damp electrode wicks by
gently lifting the IPG strip and placing it between the electrode and the strip. (Refer to Figure 9.)
Fig. 9. Electrode wicks added to a focusing tray with IPG strips already in place.
If a rehydration/equilibration tray is used for the rehydration step place the damp electrode
wicks on the electrodes of the focusing tray first (Figure 10a). Then carefully transfer the
strips from the rehydration/equilibration tray placing them gel side down onto the
electrode wicks in the channel of the focusing tray. (Figure 10b) Overlay each strip with
mineral oil or a similar overlay material.
10a. Positioning Electrode Wick 10b. Positioning ReadyStrip IPG Strip
Fig. 10. Electrode wicks and IPG strips added to a focusing tray.
Sample application if the protein sample was not included in the rehydration buffer.
The rehydrated ReadyStrips are positioned in the focusing tray and covered with mineral
oil or similar overlay material. The sample is then added to the sample loading wells on
the channel in the focusing tray. The recommended sample volume for tray loading is
up to 30 µl/ReadyStrip.
Fig. 11. Sample Application.
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