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Bio-Rad PROTEAN® IEF Cell Accessories User Manual

Page 42

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Problem

Cause

Solution

Burning of strips

• Current limit too high.

• We recommend a current limit

(this will cause large

50 µA/strip. Make sure the

fluctuations in

correct number of IPG strips

resistance resulting

was entered.

in the resistance error

• IPG strips have dried out.

• Make sure IPG strips are

message.)

covered with mineral oil or
equivalent to prevent drying
out

• Electrode wicks too wet

• Make sure the electrode wicks

or contain incorrect

contain de-ionized water only

electrode solution.

and are damp, not wet.

• Rehydration buffer

• Check rehydration buffer

composition incorrect,

concentration. Remake buffer if

salt concentration too high.

if necessary.

Section 12
Accessories

12.1 Optional Thermal Printer

Thermal Printer can be directly used with the PROTEAN IEF Cell and comes ready to

connect to the RS232 serial port. A straight, serial cable is included with the printer and the
DIP switches are pre-set to match the PROTEAN IEF Cell requirements.

Configuration

The RS232 serial port

Signal and Direction

Thermal Printer

Pin 2

Transmit

Pin 2

Pin 3

Receive

Pin 3

Pin 5

Ground

Pin 5

other pins

Not connected

other pins

Thermal Printer

1. Insert the DC plug on the AC adaptor into the power supply jack on the printer.

2. Plug the AC adaptor into an outlet.

3. Connect the straight, serial cable to the RS232 port of the PROTEAN IEF Cell and to

the serial port located on the back of the printer.

4. Load the thermal paper into the printer (refer to the Printer Manual).

5. The printer DIP switches have been preset to match the requirements of the PROTEAN

IEF Cell.

To print the current DIP switch settings proceed as follows:

a.

Slide the power switch to ON while pressing the ONLINE button.

b.

Release the ONLINE button and a list of the current settings will be printed. The
completed printout is followed by the prompt
"Continue?: Push On-line SW"
"Write?: Push paper feed SW"

c.

Push the Feed button to return to ONLINE Mode.

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