Bio-Rad PROTEAN® IEF Cell Accessories User Manual
Page 23
Section 6
Programming a New Method
In the NEW METHOD mode a method containing one (1) to ten (10) steps can be
programmed. Each method allows programming of the following parameters:
A rehydration step (optional)
•
passive or active at 50 V
•
rehydration temperature (10–25 °C)
•
rehydration time up to 99:00 hours
A current limit up to 99 µA per strip. 50 µA per strip is recommended to prevent IPG
strip overheating.
The isoelectric focusing temperature (10–25 °C)
Ten separate steps each programmable for:
•
Voltage
•
Voltage ramping slope (rapid, linear, or slow)
•
Time in hours:minutes or volt-hours.
20
See also other documents in the category Bio-Rad Accessories for water:
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- ChemiDoc™ MP System (8 pages)
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- Image Lab™ Software (260 pages)
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- ReadyPrep™ 2-D Starter Kit (28 pages)
- Ready Gel® Zymogram Precast Gels (46 pages)
- Mini-PROTEAN 2-D Electrophoresis Cell (44 pages)
- Precision Plus Protein™ Prestained Standards (3 pages)
- Precision Plus Protein™ Unstained Standards (16 pages)
- Prestained SDS-PAGE Standards (3 pages)
- Unstained SDS-PAGE Standards (3 pages)
- Silver Stains (20 pages)
- Biotinylated Standards (3 pages)
- Biotinylated Standards (11 pages)
- IEF and 2-D Standards (3 pages)
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- Mini-PROTEAN® Tetra Handcast Systems (10 pages)
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