Bio-Rad PROTEAN® IEF Cell Accessories User Manual

Page 26

6. The first line displays the step number, the

selected slope, and the entered voltage.
Select the time or volt-hours option for this step.
Enter a time up to 99:00 hours:minutes, or volt-
hours up to 99,999.
Press the NEXT key to continue.

7. The ADD(EDIT)/Insert/Delete screen with the

next step number in the method is displayed
Use the adjacent soft key to edit, insert, or
delete a step in the method
Press the NEXT key and repeat steps 5 and 6
to add steps to the method.
Select the method complete softkey when
programming is complete.
Note: An inserted step is inserted ahead of the
step # entered.

8. The save method screen is displayed after the

Method Complete key is selected. Select yes to
save the method to memory. Select no to run
the method without saving it to memory.

Press the NEXT key to continue.

9. The adjacent screen is displayed after saving

the method.

Enter the number of gels and edit current limit
per gel if needed to 99 µA per gel maximum.
Edit Focusing temperature if desired. Press
to start the run.

10. The Method Name Already Exists

If the method name already exists the adjacent
screen will be displayed.

Press the adjacent soft key to replace or rename
the method.

Press cancel save to return to the previous
screen.

11. Press the adjacent soft key to re-name the

method. When the cursor and enter symbols are
visible, use the alphanumeric keypad to enter
the new name.

Select the YES option to save the re-named
method. Select NO to continue to the run screen
without saving the method.

Press the NEXT key to continue to the run
screen.

12. The adjacent screen is displayed after replacing

the method. Enter the # of gels (IPG strips);
1–24 for 7 cm gels, 1–12 for 17 cm gels.

Press

to start the run and refer to Section 8

for program status screens.

S 01

xxxxxV

HRS:MIN

vhours

ENTER TIME:

00:00

ADD

(EDIT) insert delete >

ENTER STEP #:

# >

METHOD COMPLETE

METHOD NAME"

SAVE METHOD? YES

METHOD NAME EXISTS!

REPLACE

RENAME

CANCEL SAVE

ENTER # OF GELS:

1 >

µA LIMIT/GEL:

50 >

FOCUS TEMP:

20 °C >

PRESS

TO START