Bio-Rad PROTEAN® IEF Cell Accessories - Manual (Page 11)

b. Peltier Platform Temperature
The Peltier platform temperature can be controlled during the rehydration and
focusing steps. The programmable temperature range is from 10–25 °C.
c. Power Parameters
Voltage Ramping Method
Three voltage ramping options are available; Rapid, Linear and Slow. In the rapid
ramp method the voltage ramp is current limited. In the linear ramp method the
voltage increases in a linear fashion. In the slow ramp method, the voltage change
is based on a delayed voltage ramping algorithm. In all three methods the current will
not exceed the programmed current limit.
Voltage
In user defined programs, a maximum voltage value is entered for each step of the
program. The maximum voltage is 10,000V.
Focusing Time
The length of time voltage is applied can be set in units of time up to 99:00
hours:minutes, or in volt-hours up to 99,999 volt-hours.
Current Limits
The maximum current output of the PROTEAN IEF Cell is 2.4 mA. The maximum
current limit is 99 µA per strip. In the pre-set methods the default current limit is set to
50 µA to prevent overheating or burning of the IPG strips.
Hold Step
The pre-set program mode provides an optional hold step. The hold step maintains
the voltage at 500 V until the run is stopped by the user. The hold step is used to
eliminate effects of over-focusing or to prevent protein drift.
Note: A hold step can be programmed in a user-defined method by programming the
last step of the method at 500 V for a specified length of time in the event that you are not
present at the end of the focusing step.
2.2.2. Programming Instructions
The PROTEAN IEF Cell is designed to provide simple intuitive screen layouts for
easy programming.
Turn on the power switch located on the right side of the cell. The initialization screen will
be displayed for several seconds.
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SLOW RAMP
LINEAR RAMP
RAPID RAMP
VOLTAGE
TIME/VH
Max V