Bio-Rad PROTEAN® IEF Cell Accessories - Manual (Page 44)

RS232 Printout of Running Conditions
The PROTEAN IEF Cell is configured to output the running conditions every 5 minutes
during the isoelectric focusing portion of a method. The printout lists the details of the pro-
grammed method and values for all the parameters of each step.
Bio-Rad Laboratories
PROTEAN IEF Cell
Firmware Version: X.XX
Method "Name"
Rehydration: Inactive (Passive or Active @ 50 V)
Rehyde time: 00:00
Temp: 20 °C
Run Temp: 20 °C
Number of Gels: #
Max µA/Gel: 50
Step 01 250 V Ramp:R Time: 00:15
Step 02 10000 V Ramp:S Time: 01:00
etc.
End of Method list.
Start of run data:
00:00 1 xxxxV / 50 10 µA 0000 V hrs
Total time Step # Voltage and Total Current Total
elapsed Ramp type Current per gel Volthours
The printout will also indicate interruptions of the run, such as pause mode and interruption
of power when the cell cover is opened. If there is poor contact between one of the IPG strips
and the electrode, large fluctuations in resistance will hold the voltage constant at 500 volts
until the user intervenes, preventing the IPG strips from burning. If the method is edited
during the run the printout will display edited values with an asterisk.
12.2 ReadyStrips IPG Strips
ReadyStrips IPG strips are made with buffering acrylamide derivatives that contain either
a free carboxylic acid or a tertiary amino group which are co-polymerized with acrylamide and
Bis. As such, the pH gradient is precast into the gel and cannot shift during electrophoresis.
The pre-cast IPG provides reproducible gradients in an easy to use format.
Specifications 7 cm ReadyStrips 17 cm ReadyStrips
Strip dimensions
Total strip length 7.9 cm 17.8 cm
Gel length 7.25 cm 17.1 cm
Strip width 3.3 cm 3.3 cm
Gel thickness 0.5 mm 0.5 mm
Linear pH gradient ranges
Standard broad ranges 3-10, 4-7 3-10, 4-7
Overlapping, narrow ranges 3-6, 5-8, 7-10 3-6, 5-8, 7-10
Gel composition 4%T/3%C 4%T/3%C
Storage -20 °C -20 °C
Number of ReadyStrips/package 12 12
Anode (acidic) end identified by: + +
Refer to Section 15 for ordering information.
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