Bio-Rad PROTEAN® IEF Cell Accessories - Manual (Page 13)

Section 3
Rehydration Program
The rehydration mode is used to program a separate rehydration step. The IPG Strips can
be rehydrated in a temperature controlled environment. There are two different rehydration
methods available, passive and active. When using the active rehydration method the IPG
strips are positioned in the focusing tray (see Section 10 for details). For passive rehydration
the IPG strips can be positioned in either the rehydration/equilibration tray or the focusing
tray.
3.1 Rehydration Programming Diagram (not integral)
10
BIO-RAD LABORATORIES
PROTEAN IEF CELL
FIRMWARE VERSION 1.40
SYSTEM INITIALIZATION
**CELL COVER OPEN**
REHYDRATION PAUSED.
CLOSE COVER
TO PROCEED.
REHYDRATION >
PRESET METHOD >
STORED METHOD >
NEW METHOD >
REHYDRATION CONDITION
active @50V PASSIVE >
ENTER TEMP:
20 °C >
PRESS TO START
REHYDRATION CONDITION
ACTIVE @50V passive
>
ENTER TEMP: 20 °C >
PRESS TO START
REHYDRATION: PASSIVE
TEMP: 20/20 °C
TIME ELAPSED: 00:00
PRESS
TO EXIT
REHYDRATION: AT 50V
TEMP: 20/20 °C
TIME ELAPSED: 00:00
PRESS
TO EXIT
START
KEY
START
KEY
STOP
KEY
STOP
KEY
STOP
KEY
EXIT
KEY
&