LaMotte SMART Spectro Spectrophotometer - Manual (Page 58)

AMMONIA-NITROGEN–LOW RANGE
SALICYLATE METHOD • CODE 3659-01-SC
QUANTITY CONTENTS
CODE
60 mL *Salicylate Ammonia #1 *3978-H
10 g *Salicylate #2 *7457-D
2 x 5 g *Salicylate #3 *7458-C
1 Spoon, 0.1 g, plastic 0699
1 Spoon, 0.15 g, plastic 0727
1 Pipet, 1.0 mL, plastic 0354
*WARNING: Reagents marked with a * are considered to be potential health hazards.
To view or print a Material Safety Data Sheet (MSDS) for these reagents see MSDS
CD or www.lamotte.com. To obtain a printed copy, contact LaMotte by e-mail, phone
or fax.
Ammonia nitrogen is present in various concentrations in many surface and
ground water supplies. Any sudden change in the concentration of ammonia
nitrogen in a water supply is cause for suspicion. A product of microbiological
activity, ammonia nitrogen is sometimes accepted as chemical evidence of
pollution when encountered in natural waters.
Ammonia is rapidly oxidized in natural water systems by special bacterial
groups that produce nitrite and nitrate. This oxidation requires that dissolved
oxygen be available in the water. Ammonia is an additional source of nitrogen
as a nutrient which may contribute to the expanded growth of undesirable
algae and other forms of plant growth that overload the natural system and
cause pollution.
APPLICATION: Low concentrations of ammonia in fresh, brackish and salt
water; fresh and salt water aquariums.
RANGE: 0.00–1.00 ppm Ammonia-Nitrogen
METHOD: Salicylate and ammonia react at high pH in the presence of a
chlorine donor and an iron catalyst to form a blue
indophenol dye, the concentration of which is proportional
to the ammonia concentration in the sample.
SAMPLE
HANDLE &
PRESERVATION: Ammonia solutions tend to be unstable and should be
analyzed immediately. Samples may be stored for 24 hours at
4°C or 28 days at –20°C.
INTERFERENCES: There are few interferences in most natural waters. High
concentrations of reducing agents, such as hydrazine, react
with the chlorine donor and can result in negative
interferences. Color and turbidity can also interfere.
SMART SPECTRO TEST PROCEDURES 05.04 Ammonia-Nitrogen LR 1/3