LaMotte SMART Spectro Spectrophotometer - Manual (Page 155)

PROCEDURE
Use universal sample holder.
1. Press and hold
ON button until spectrophotometer turns on.
2. Scroll to and select PROGRAMMED TESTS.
3. Scroll to and select ALL TESTS (or another sequence containing
53 Iron Phen
) from TESTING MENU.
4. Scroll to and select 53 Iron Phen from menu.
5. Rinse a clean tube (0290)with sample water. Fill to the 10 mL mark with
sample.
6. Insert tube into chamber, close lid and select SCAN BLANK.
7. Remove the tube from Spectro. Remove the cap and add 6 drops of *Acid
Phenanthroline Indicator (2776). Cap and invert the tube 4 times to mix
reagents. Wait five minutes for maximum color development.
8. After five minutes, mix, insert tube into chamber, close lid and select
SCAN SAMPLE
. Record result as ppm Ferrous Iron.
9. Remove tube from Spectro. Use the 0.1g spoon (0699) to add one measure
of *Iron Reducting Reagent (2777). Cap and invert 15-20 times to mix,
wait 5 minutes for maximum color delelopment.
10. After 5 minutes, mix insert tube into Spectro. Close lid and select SCAN
SAMPLE.
Record result as ppm Total Iron.
11.. Press
OFF button to turn spectrophotometer off or press EXIT button to
exit to a previous menu or make another menu selection.
12. Total Iron (ppm) - Ferrous Iron (ppm) = Ferric Iron (ppm)
þNOTE: For best possible results, a reagent blank should be determined to
account for any contribution to the test result by the reagent system. To
determine the reagent blank, follow the above test procedure to scan a
distilled or deionized water blank. Then follow the above procedure to
perform the test on a distilled or deionized water sample. This test result is
the reagent blank. Subtract the reagent blank from all subsequent test results
of unknown samples. It is necessary to determine the reagent blank only
when a new lot number of reagents are obtained.
Iron–I,I0-Phenanthroline Method 2/2 SMART SPECTRO TEST PROCEDURES 01.07