LaMotte SMART Spectro Spectrophotometer - Manual (Page 145)

PROCEDURE
Use universal sample holder.
1. Press and hold
ON button until spectrophotometer turns on.
2. Scroll to and select PROGRAMMED TESTS.
3. Scroll to and select ALL TESTS (or another sequence containing
45 Hydrazine
) from TESTING MENU.
4. Scroll to and select 45 Hydrazine from menu.
5. Rinse a clean tube (0290) with sample water. Fill to the 10 mL line with
sample.
6. Insert tube into chamber, close lid and select SCAN BLANK.
7. Remove tube from Spectro. Use the 1 mL pipet (0354) to add 4 mL of
*Hydrazine Reagent A (4841). Cap and mix.
8. Use the 0.15 g spoon (0727) to add one measure of *Hydrazine Reagent B
Powder (4842). Cap and shake vigorously for 10 seconds. Wait 2 minutes
for maximum color development. An undissolved portion of Hydrazine
Reagent B may remain in bottom of tube without adversely affecting
results.
9. At the end of the 2 minute waiting period, mix, insert tube into chamber,
close lid and select SCAN SAMPLE. Record result.
10. Press
OFF button to turn spectrophotometer off or press EXIT button to
exit to a previous menu or make another menu selection.
þNOTE: For best possible results, a reagent blank should be determined to
account for any contribution to the test result by the reagent system. To
determine the reagent blank, follow the above test procedure to scan a
distilled or deionized water blank. Then follow the above procedure to
perform the test on a distilled or deionized water sample. This test result is
the reagent blank. Subtract the reagent blank from all subsequent test results
of unknown samples. It is necessary to determine the reagent blank only
when a new lot number of reagents are obtained.
Hydrazine 2/2 SMART SPECTRO TEST PROCEDURES 05.04