LaMotte SMART Spectro Spectrophotometer User Manual
Page 192
PROCEDURE–HIGH RANGE
Use universal sample holder.
A. PREPARATION OF HR REAGENT
þNOTE: The quantity of Indigo Blue Stock solution (3989) supplied will
prepare one batch of HR Reagent for the High Range Ozone procedure or
five batches of HR Reagent for the Low Range Ozone procedure.
1.
Use the 50 mL graduated cylinder to carefully add 35 mL of *Ozone Buffer
(3991) to amber glass bottle marked HR Reagent (0680).
2.
Use the 50 mL graduated cylinder to carefully add 15 mL of Indigo Blue
Stock Solution (3989) to the amber glass bottle. Cap and mix.
B. DETERMINATION OF OZONE
3.
Use the 1.0 mL transfer pipet (2-2170) and pump (30527) to add 1.0 mL of
HR Reagent to each of 2 clean tubes (0290).
4.
If chlorine is present add 3 drops Chlorine Inhibitor (3990) to each tube.
Cap tubes.
5.
Take one of the prepared tubes (0290) and sampling apparatus (0681) to
sampling site.
6.
Lower end of tubing of sampling apparatus to desired depth. Slowly
withdraw and depress plunger several times to purge syringe and tubing.
Slowly withdraw plunger to fill purged syringe.
7.
Remove plastic tubing from syringe. Remove cap from the prepared tube.
Place tip of syringe against inside of the prepared tube. Slowly depress
plunger and fill to the 10 mL line and cap. This is the Sample Tube.
þNOTE: DO NOT SHAKE OR INVERT THE SAMPLE.
8.
Fill the second prepared tube (0290) to the 10 mL line with ozone free
water. This is the Reagent Blank.
9.
Press and hold
ON button until spectrophotometer turns on.
10.
Scroll to and select PROGRAMMED TESTS.
11.
Scroll to and select ALL TESTS
(or another sequence containing
72 OZONE-HR
) from TESTING MENU.
12.
Scroll to and select 72 OZONE-HR from menu.
13.
Insert the Reagent Blank tube into chamber, close lid and select SCAN
BLANK
.
14.
Insert reacted Sample Tube into chamber, close lid and select SCAN
SAMPLE
. Record result.
15.
Press
OFF button to turn spectrophotometer off or press EXIT button to
exit to a previous menu or make another menu selection.
þNOTE: HR Reagent must be made fresh each week. If reagent is
refrigerated, it may be kept up to 3 weeks.
SMART SPECTRO TEST PROCEDURES 07.05
Ozone 3/3